What causes Prozone phenomenon?
Keeping this in view, how can Prozone phenomenon be overcome?
It can be overcome by appropriate dilution and titration of antisera. We have observed that by simply washing the RBCs once more after the antiglobulin phase the prozone phenomenon is elimi- nated, and the strength of agglutination of RBCs (i.e., the sensitivity of the test) is con- siderably increased.
Also, what is Prozone and Postzone? The prozone reaction refers to the absence of antibody-antigen precipitation in the presence of antibody excess. Since the authors refer to situations of antigen excess, the term prozone cannot correctly be used. Instead, absence of precipitation with excess antigen is described as the postzone phenomenon.
Just so, what does Prozone effect mean?
The hook effect or the prozone effect is an immunologic phenomenon whereby the effectiveness of antibodies to form immune complexes is sometimes impaired when concentrations of an antibody or an antigen are very high.
What is antigen excess?
Antigen excess (also known as “prozone” or “hook effect”) occurs when antigen is present in such high levels that it limits the antigen-antibody crosslinking, resulting in the formation of smaller immune complexes causing immunoassays to underestimate high concentrations of protein (see figure below)