What is fixative used for?
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Consequently, what is the purpose of fixation?
The aim of fixation The aim of fixation is to preserve cells or tissues in as near a life like condition as possible, prevent autolysis and putrefaction, and protect the tissue from subsequent processing. Fixatives have different actions e.g. crosslinking, precipitative, coagulative etc.
Furthermore, what are the types of fixatives? Popular fixative solutions
- Phosphate buffered formalin.
- Formal calcium.
- Formal saline.
- Zinc formalin (unbuffered)
- Zenker's fixative.
- Helly's fixative.
- B-5 fixative.
- Bouin's solution.
Consequently, what is a fixative solution?
Fixative: A medium such as a solution or spray that preserves specimens of tissues or cells. Most biopsies and specimens removed at surgery are fixed in a solution such as formalin (dilute formaldehyde) before further processing takes place.
What is an ideal fixative?
An ideal fixative should: Preserve the tissue and cells as life-like as possible, without any shrinking or swelling and without distorting or dissolving cellular constituents. Prevent putrefaction by bacteria and prevent autolysis by cathepsin-containing cells.