What is cytological fixative?
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Likewise, what are fixatives?
A fixative is a stabilizing or preservative agent: Dye fixatives or mordants, are chemical substances used in processing fabrics to create circumstances in the micro-substrates causing dye molecules to adhere and remain that way.
Also, what are the qualities of a good fixative? Essential 2: Proper penetration of fixative
- Fixative should penetrate from all sides.
- Cavities should be opened.
- Perfusion of some specimens is advantageous.
- Thickness is important (4mm maximum).
- Some agitation is useful.
- An adequate volume is vital (20:1 at least).
- Allow sufficient time.
- Room temperature is best.
Subsequently, one may also ask, what fixative can be used for cytology smears?
Methanol is commonly used as a fixative for blood films and 95% ethanol is used as a fixative for cytology smears but both alcohols are usually combined with other reagents when used as fixatives for tissue specimens.
What are the types of fixatives?
Popular fixative solutions
- Phosphate buffered formalin.
- Formal calcium.
- Formal saline.
- Zinc formalin (unbuffered)
- Zenker's fixative.
- Helly's fixative.
- B-5 fixative.
- Bouin's solution.