How do you wet load a column?
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Correspondingly, why is it important not to let the stationary phase dry out when running a column?
In other words, the slurry inside the column should never be allowed to dry out. If this happens it may create cracks and unevenness in the solid phase, which will decrease the efficiency of the separation. The sample to be separated is loaded in solution in a suitable solvent, preferably as concentrated as possible.
Beside above, how much silica do you use in a column? Typically, flash chromatography purification of 1g of sample will require 20g to 100g of silica, and 200 to 1000mL of total elutant volume. If the column is successful, the desired component should be eluted in no more than 1/10 of the total elutant volume.
In respect to this, what happens if column runs dry?
If you let the column run dry the silica will start to crack and you will get poor separation of your compounds. As you run the column, never let the level of solvent go below the level of the silica gel or you will get poor results.
How do you load a column?
To load the column:
- Dissolve the sample in the minimum amount of solvent (5–10 drops).
- Using a pipette or syringe with a thick needle, drip the sample directly onto the top of the silica.
- Once the entire sample has been added, allow the column to drain so that the solvent level touches the top of the silica.