Why N 10 HCl is used in Hb estimation?

Asked By: Iman Angrill | Last Updated: 14th May, 2020
Category: medical health diabetes
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The principle of Sahli's Method or Acid hematin method is quite easy that when the blood is added to N/10 Hydrochloric acid (HCl), the hemoglobin present in RBCs is converted to acid hematin which is a dark brown colored compound.

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Correspondingly, what is the principle of Haemoglobin estimation?

This is the internationally recommended method for determining hemoglobin. Principle: Blood is diluted in a solution containing potassium cyanide and potassium ferricyanide. The latter converts Hb to methemoglobin which is converted to cyanmethemoglobin (HiCN) by potassium cyanide.

Likewise, how is acid Hematin formed? This pigment is produced by acid acting upon hemoglobin and is known as formalin pigment or acid hematin. These parasites disintegrate erythrocytes in an unknown manner, and liberate an acid hematin-like pigment which is phagocytized by the reticuloendothelial system.

Also question is, why only HCl is used in Sahli's method?

Concentrated acid if used in Sahli's method gives always the same colour for different hemoglobin concentrations, hence it can't be used for hemoglobin estimation.

What is the principle of Sahli's method?

Principle The Sahli method is based on converting hemoglobin to acid hematin and then visually matching its color against a solid glass standard. Dilute hydrochloric acid is added to a graduated cylinder containing a blood sample until the color of the diluted blood sample matches that of the glass standard.

23 Related Question Answers Found

How many types of Hb estimation are there?

Samples were analyzed using four different methods of Hb estimation: Automated hematology cell analyzer (ABX Micros 60, France), HCS, CuSO4 specific gravity method and Hemocue (Hemocue B - hemoglobin photometer; Angelholm, Sweden).

What are normal Hb levels?

The normal range for hemoglobin is: For men, 13.5 to 17.5 grams per deciliter. For women, 12.0 to 15.5 grams per deciliter.

What are the 3 types of hemoglobin?

There are several different types of globin chains, named alpha, beta, delta, and gamma. Normal hemoglobin types include: Hemoglobin A (Hb A): makes up about 95%-98% of hemoglobin found in adults; it contains two alpha (α) chains and two beta (β) protein chains.

How do you measure hemoglobin?

Hemoglobin is routinely measured as part of a routine blood test termed blood count (CBC). A complete blood count is one of the most common tests doctors order for a patient. It is done on automated machines that use a blood sample that is chemically treated to release hemoglobin from red blood cells.

What are the functions of hemoglobin?


Hemoglobin is contained in red blood cells, which efficiently carries oxygen from the lungs to the tissues of the body. Hemoglobin also helps in the transportation of carbon dioxide and hydrogen ions back to the lungs.

How do I raise my hemoglobin?

increasing the intake of iron-rich foods (eggs, spinach, artichokes, beans, lean meats, and seafood) and foods rich in cofactors (such as vitamin B6, folic acid, vitamin B12, and vitamin C) important for maintaining normal hemoglobin levels. Such foods include fish, vegetables, nuts, cereals, peas, and citrus fruits.

What does the Tallquist method determine?

The Tallquist method makes possible a quick assessment of a patient's hemoglobin levels. A drop of blood is absorbed into a test paper, and the colour of the blood is compared to the colours on a chart. The colours correspond to different concentrations of hemoglobin. This tool was formerly used to diagnose anemia.

What is Hematin used for?


Uses. This medication is used to treat the symptoms that occur with certain blood disorders (porphyrias). Other treatments are recommended for trial before using hemin, also called hematin. Hemin is not a cure for porphyria.

What is Sahli's Haemoglobinometer?

Hemoglobinometer. an instrument that is used to determine the quantity of hemoglobin in the blood. In practice the hemoglobinometer proposed in 1902 by the Swiss scientist H. Sahli is used. It is based on comparison of the color of the tested blood, which is treated with hydrochloric acid, with the color of standards.

What is Haemometer?

A hemoglobinometer is a medical measuring device of hemoglobin blood concentration. It can operate by spectrophotometric measurement of hemoglobin concentration. Portable hemoglobinometers provide easy and convenient measurement of hematological variables, especially in areas where no clinic laboratories are available.

What is Cyanmethemoglobin method?

In the Drabkin's method of haemoglobin estimation haemoglobin is oxidised to methemoglobin by potassium ferricyanide, which reacts with cyanide ions of potassium cyanide to form cyanmethemoglobin. The haemoglobin is estimated with the help of cyanmethemoglobin curve.

What is formalin pigment?

Formalin pigment is a brown, granular, doubly refractile deposit seen both intracellularly and extracellularly in tissues which have been fixed with a simple formalin solution, such as formal-saline. It is also known as acid formaldehyde hematin, as it is formed from hemoglobin by the action of formaldehyde at acid pH.

What is beta Hematin?


beta-hematin. Definition. A dimer of hematin in which an iron-oxygen bond links the central iron of one hematin to the oxygen of one of the carboxylates of the adjacent hematin.

Which meniscus of acid Hematin is considered?

Lower meniscus of acid hematin describes the phase boundary between acid hematin and air.

What are the advantages and disadvantages of Sahli's method?

Advantages and Disadvantages of Sahli's acid hematin method
Advantages Disadvantages
Easy to perform Less accurate
Quick All hemoglobins (oxyhemoglobin, sulfhemoglobin) are not converted to acid hematin and hence the value of Hb obtained is less than the actual value.
Inexpensive The color of acid hematin develops slowly.