What is the dilution of blood during count?
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Also asked, how is a Hemocytometer used to count red blood cells?
Counting. Using a pipette, take 100 µL of Trypan Blue-treated cell suspension and apply to the hemocytometer. If using a glass hemocytometer, very gently fill both chambers underneath the coverslip, allowing the cell suspension to be drawn out by capillary action.
Subsequently, question is, what magnification is used to count red blood cells? Using light microscopy, imaging is done with a 10x ocular and 10x objective at first. Increasing in progression, it is best to view smears up to a total magnification of 1000x using the 10x ocular. Red blood cells will stain pink, platelets appear as small blue/purple and cytoplasmic granules stain pink to violet.
Furthermore, what does the red blood cell diluting fluid consist of?
Hayem's is common reagent that used in red blood cell counting. it consist of HgCl2, NaSO4 and NaCl.
What is a normal red blood cell count?
According to the Leukemia & Lymphoma Society: The normal RBC range for men is 4.7 to 6.1 million cells per microliter (mcL). The normal RBC range for women who aren't pregnant is 4.2 to 5.4 million mcL. The normal RBC range for children is 4.0 to 5.5 million mcL.