What is RFLP and how is it used?
Also to know is, what are RFLPs used for?
Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes. These are bacterial enzymes used by scientists to cut DNA molecules at known locations. RFLPs (pronounced "rif lips") are used as markers on genetic maps.
Subsequently, question is, what do you mean by RFLP? Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases.
Also to know, how do you perform RFLP?
Step-by-Step Guide to RFLP Analysis
- Step 1 Isolate DNA.
- Step 2 Perform PCR.
- Step 3 Perform Restriction Digestion.
- Step 4 Prepare Sample for Analysis.
- Step 5 Perform Capillary Electrophroesis.
- Step 6 Analyze Data.
Which enzyme is used in RFLP process?
In RFLPs the DNA is cut using a restriction enzyme, an enzyme that cuts the DNA only where it recognizes a specific sequence of nucleotides (a restriction site). The pieces vary in size based on the location of the restriction sites.