What is ChIP Chip assay?
Category:
science
genetics
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1,2). In addition, the ChIP assay can be used to define the spatial and temporal relationship of a particular protein-DNA interaction.
Simply so, what is ChIP analysis?
ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.
Also know, what is ChIP data?
Chromatin immunoprecipitation (ChIP) allows us to determine protein-binding sites on DNA. Chromatin is the complex of DNA packaged with histone proteins into nucleosomes. ChIP makes use of reversible cross-links made between DNA and associated proteins by formaldehyde fixation of cells or tissue.
Analysis of ChIP-seq data
- pre-process sequencing reads.
- map reads.
- post-process mapped data.
- assess quality and strength of ChIP-signal.
- display coverage plots in a genome browser.
- call ChIP peaks with MACS2.
- inspect obtained calls.
- look for sequence motifs within called peaks.