What factors are involved in the separation of pigments?
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science
chemistry
The factors involved in the separation of pigments are the solvent and the amount of hydrogen bonding the pigment has to the cellulose. Chlorophylls contain oxygen and nitrogen bonds, which hold more tightly to the paper; it does not make up as much as carotene, which does not form hydrogen bonds.
People also ask, what factors are involved in the separation of the pigments quizlet?
Solubility, the size of the particles and the pigments attractiveness to the paper based upon the pigment's chemical structure.
Considering this, why do pigments separate in chromatography?
The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent. The solvent carries the dissolved pigments as it moves up the paper. The pigments are carried at different rates because they are not equally soluble.
Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate.