What factors are involved in the separation of pigments?
People also ask, what factors are involved in the separation of the pigments quizlet?
Solubility, the size of the particles and the pigments attractiveness to the paper based upon the pigment's chemical structure.
Furthermore, how would the Rf value for each pigment change? Based on the Rf values, xanthophylls are more soluble in the chromatography solvent. If altering the composition of the solvent resulted in a change the solubility of the pigment in the solvent, the Rf value would also change.
Considering this, why do pigments separate in chromatography?
The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent. The solvent carries the dissolved pigments as it moves up the paper. The pigments are carried at different rates because they are not equally soluble.
What factors affect chromatography?
Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate.