What factors are involved in the separation of pigments?

Asked By: Madelaine Yanko | Last Updated: 13th June, 2020
Category: science chemistry
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The factors involved in the separation of pigments are the solvent and the amount of hydrogen bonding the pigment has to the cellulose. Chlorophylls contain oxygen and nitrogen bonds, which hold more tightly to the paper; it does not make up as much as carotene, which does not form hydrogen bonds.

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People also ask, what factors are involved in the separation of the pigments quizlet?

Solubility, the size of the particles and the pigments attractiveness to the paper based upon the pigment's chemical structure.

Furthermore, how would the Rf value for each pigment change? Based on the Rf values, xanthophylls are more soluble in the chromatography solvent. If altering the composition of the solvent resulted in a change the solubility of the pigment in the solvent, the Rf value would also change.

Considering this, why do pigments separate in chromatography?

The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent. The solvent carries the dissolved pigments as it moves up the paper. The pigments are carried at different rates because they are not equally soluble.

What factors affect chromatography?

Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate.

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What are functions of other pigments?

The primary function of pigments in plants is photosynthesis, which uses the green pigment chlorophyll and several colorful pigments that absorb as much light energy as possible. Other functions of pigments in plants include attracting insects to flowers to encourage pollination.

What is the function of DPIP in this experiment?

The function of the DPIP is to change from blue to clear as photosynthesis proceeds and DPIP is reduced, allowing the spectrometer to measure the rate of photosynthesis through the clarity of the DPIP solution. The DPIP replaces the NADP+ molecules in photosynthesis. The source of the electrons is the water. 4.

What are the different photosynthetic pigments?

There are five main types of chlorophylls: chlorophylls a, b, c and d, plus a related molecule found in prokaryotes called bacteriochlorophyll. In plants, chlorophyll a and chlorophyll b are the main photosynthetic pigments.

What is the function of photosynthetic pigments?

Because they interact with light to absorb only certain wavelengths, pigments are useful to plants and other autotrophs --organisms which make their own food using photosynthesis. In plants, algae, and cyanobacteria, pigments are the means by which the energy of sunlight is captured for photosynthesis.

What is the role of chlorophyll and other pigments in the reaction center?


The function of the reaction center chlorophyll is to use the energy absorbed by and transferred to it from the other chlorophyll pigments in the photosystems to undergo a charge separation, a specific redox reaction in which the chlorophyll donates an electron into a series of molecular intermediates called an

Which pigments form hydrogen bonds with cellulose?

Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Another pigment, xanthophylls, differs from carotene in that it contains oxygen.

Would you expect the RF value of a pigment to be the same if a different solvent were used?

If a different solvent were used, the Rf value would not be the same because, depending on the solvent, there is a difference in solubility. If a different solvent was used, the relative positions of the bands would be different. What type of chlorophyll does the reaction center contain?

What is Rf value?

The Rf value is defined as the ratio of the distance moved by the solute (i.e. the dye or pigment under test) and the distance moved by the the solvent (known as the Solvent front) along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is

What do you mean by stationary phase?


stationary-phase. Noun. (plural stationary phases) (chemistry) The solid or liquid phase of a chromatography system on which the materials to be separated are selectively adsorbed.

What color is Xanthophyll after separation?

The top band of pigments in the separation are carotenoids called carotenes, most likely beta-carotene, and appear yellowish-orange. The second type of carotenoid separated in the experiment are xanthophylls, which appear bright yellowish and are most likely lutein.

How do you extract photosynthetic pigments?

Extract photosynthetic pigments by grinding 2g of your leaves, torn into small pieces, in a mortar with a pinch of clean sand and a total of 10mL of 100% acetone. Initially, add only a small amount of acetone to begin the grinding process. It is much easier to grind the leaves if the extract is a pasty consistency.

How do you extract plant pigments?

Procedures
  1. Obtain a mortar and pestle and choose a plant for your extraction.
  2. Add the plant to the mortar and then crush your plant in the mortar with the pestle.
  3. Crush as much as you can and then add the solvent (Water, Acetone and or Ethanol).
  4. Filter the extract to remove all non-soluble plant material.

Which pigment has the highest RF value?

Pigment Rf value range Relative position
Lutein 0.22-0.28 Below, or almost at the same level of, the highest green
Violaxanthin 0.13-0.19 Below, or almost at the same level of, the highest green
Neoxanthin 0.04-0.09 Below, or almost at the same level of, the highest green

Which pigments are most soluble in chromatography solvent?


The orange colored band, made of the pigment called carotenoids. is the most soluble in alcohol, so it traveled the farthest. The yellow xanthophylls are the next most soluble, followed by the blue-green chlorophyll A. The least soluble pigment is the yellow green chlorophyll B.

Why are two solvents used in chromatography?

Chromatography is a technique used to separate the components of a mixture. Different solvents will dissolve different substances. A polar solvent (water) will dissolve polar substances (water soluble ink in the video below). A non-polar solvent will dissolve non-polar substances.