What does reticulin stain for?

The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens. Additionally, fibrosis can be noted on bone marrow specimens that have significant tumor metastasis.

Keeping this in view, what is the purpose of special stains?

"Special stains" are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. They allow the presence/or absence of certain cell types, structures and/or microorganisms to be viewed microscopically.

Subsequently, question is, what is reticulin fibrosis? Increased reticulin staining (reticulin fibrosis) is associated with many benign and malignant conditions while increased trichrome staining (collagen fibrosis) is particularly prominent in late stages of severe myeloproliferative diseases or following tumour metastasis to the bone marrow.

Likewise, where is Reticulin found?

Reticular fibers crosslink to form a fine meshwork (reticulin). This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system.

What stain is used for elastic fibers?

Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG), is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 1908. The formulation is used to demonstrate normal or pathologic elastic fibers.

35 Related Question Answers Found

Why is H&E staining important?

Uses. The H&E staining procedure is the principal stain in histology in part because it can be done quickly, is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy is revealed, and can be used to diagnose a wide range of histopathologic conditions.

What are the types of staining?

A variety of staining techniques can be used with light microscopy, including Gram staining, acid-fast staining, capsule staining, endospore staining, and flagella staining.

What are the special stains for fat?

Fat stains

The oil red O (ORO) stain can identify neutral lipids and fatty acids in smears and tissues. Fresh smears or cryostat sections of tissue are necessary because fixatives containing alcohols, or routine tissue processing with clearing, will remove lipids. The ORO is a rapid and simple stain.

How does trichrome stain work?

Trichrome staining techniques employ two or more acid dyes. Normally acid dyes would stain the same basic proteins, but by applying them sequentially the staining pattern can be manipulated. A polyacid (such as phosphomolybdic acid or tungstophosphoric acid) is used to remove dye selectively.

What is histology staining?

Staining is used to highlight important features of the tissue as well as to enhance the tissue contrast. Hematoxylin is a basic dye that is commonly used in this process and stains the nuclei giving it a bluish color while eosin (another stain dye used in histology) stains the cell's nucleus giving it a pinkish stain.

What two stains are used to identify lipids?

Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics (plastic colorants) and are also used to stain sudanophilic biological samples, usually lipids.

Why do we stain tissue?

The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.

What is a histochemical stain?

Introduction. Histochemistry is an important technique that is used for the visualization of biological structures. As such, it is concerned with the identification and distribution of various chemical components of tissues through the use of stains, indicators as well as microscopy.

What is the function of connective tissue?

Major functions of connective tissue include: 1) binding and supporting, 2) protecting, 3) insulating, 4) storing reserve fuel, and 5) transporting substances within the body. Connective tissues can have various levels of vascularity. Cartilage is avascular, while dense connective tissue is poorly vascularized.

Where is dense regular connective tissue found?

Dense regular connective tissue

It is found in areas of the body where large amounts of tensile strength are required, like in ligaments, tendons and aponeurosis. The collagen fibers are densely packed together and arranged in parallel to each other.

Is blood a connective tissue?

Blood is considered a connective tissue because it has a matrix. The living cell types are red blood cells, also called erythrocytes, and white blood cells, also called leukocytes. The fluid portion of whole blood, its matrix, is commonly called plasma.

Where are fibroblasts found?

Introduction. Fibroblasts are typically spindle-shaped cells with an oval flat nucleus found in the interstitial spaces of organs. In the lung, they reside in highly complex multicellular environments, usually closely apposed to the epithelium or endothelium.

What are three types of fibers?

Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers.

What are the connective tissue?

Connective tissue: A material made up of fibers forming a framework and support structure for body tissues and organs. Connective tissue surrounds many organs. Cartilage and bone are specialized forms of connective tissue. All connective tissue is derived from mesoderm, the middle germ cell layer in the embryo.

Are reticular fibers elastic?

Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column. Reticular fiber is also formed from the same protein subunits as collagen fibers; however, these fibers remain narrow and are arrayed in a branching network.

Are elastic fibers made of collagen?

Elastic fibers are composed of elastin and proteins, often referred to as microfibrils, such as fibrillin, because their fibrils are much smaller than the classic collagen fibrils. Elastic fibers must withstand repeated stretching and deformation and must be capable of returning to a relaxed state.

What is reticulin in bone marrow?

The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens. Additionally, fibrosis can be noted on bone marrow specimens that have significant tumor metastasis.