What does bapna indicate?
Consequently, what is the purpose of bapna?
BAPNA is a “fake”/placebo substrate which released a dye when hydrolyzed. The Spectrometer's purpose was to measure the amount of dye that was released/produced.
Furthermore, why is bapna used as an artificial substrate for trypsin? Trypsin has a much higher affinity for Bz-Phe-Val-Arg-pNA than for benzoyl-DL-arginine p-nitroanilide (BAPNA). The synthetic peptide substrates are useful for determination of enzymes of the trypsin type, and they can also be used in biological fluids. Small amounts of trypsin, thrombin and Reptilase can be determined.
Considering this, what was pepsin effect on bapna?
The substrate is BAPNA, a synthetic peptide, and pepsin usually hydrolyzes polypeptides found in food. The presence of pepsin hydrolyzes polypeptides into peptides and free amino acids.
Why does the pH change in the test tube in which fat digestion occurs?
Explain why fat digestion affects the pH of the solution. Fats have a neutral pH. However, when digested by lipase fats are converted into fatty acids. The presence of fatty acids can decrease the pH or increase the acidity of the solution (hence the name fatty ACID).