How do you perform a 10 fold serial dilution?
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Then, what does it mean by 10 fold dilution?
A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. A series of ten-fold dilutions is described as ten-fold serial dilutions.
Furthermore, what is a 5 fold serial dilution? “The dilution factor is 5" “It was a 5 fold dilution” “It was diluted 1/5" These all mean the same thing, that there is 1 volume part of sample and 4 volume parts of whatever liquid is being used to dilute the sample for a total of 5 volume parts. CALCULATING THE CONCENTRATION.
Additionally, how do you do a two fold serial dilution?
So, make three serial 1/10 dilutions (0.1 ml [100 microliters] into 0.9 ml): 1/10 x 1/10 x 1/10 = 1/1,000. Now you could add 1.0 ml of the starting 1/1,000 dilution to 1.0 ml of diluent, making a 2-fold dilution (giving 1/2,000).
Why is serial dilution performed?
A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. Each dilution will reduce the concentration of bacteria by a specific amount.