How do you make a KOH solution?
- Weigh 20 g potassium hydroxide (KOH)pellets.
- Transfer the chemical to a screw-cap bottle.
- Add 50 ml distilled water, and mix until the chemical iscompletely dissolved, add remaining distilled water and makethe volume 100 ml.
- Label the bottle and mark it corrosive.
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Thereof, what is the purpose of 20% KOH?
SUMMARY. Potassium Hydroxide (KOH)Solutions are used in a rapid method for distinguishing fungalelements in microscopic preparations. KOH Solutions areuseful when examining mucoid specimens or material containingkeratin, such as skin, scales, nails, or hair.
Secondly, how does the KOH test work? General. The purpose of the potassium hydroxidetest (KOH test) is to identify gram negative bacteria.Disintergration of gram negative cell walls lyses the cell andrelease its contents, including the DNA. The DNA will make thesolution very viscous and the solution will stick to the plasticloop when touched.
Keeping this in view, how do you make methanolic KOH?
Dissolve about 6 g of potassium hydroxide in 5 mlof water. Add sufficient aldehyde-free ethanol (95 %) toproduce 1000 ml. Allow the solution to stand in atightly-stoppered bottle for 24 hours. Then quickly decant theclear supernatant liquid into a suitable, tightly-closedcontainer.
What is the purpose of using KOH in the microscopic examination of fungi?
Wood lamp examination It can be used to detect fungal infections inthe hair or scalp, also known as tinea capitis. If a fungalinfection is present, the sample will glow a greenish or bluishcolor, making it easy to see and diagnose.